HMW glutenin composition in wheat doubled haploid populations
نویسندگان
چکیده
منابع مشابه
Generation of Doubled Haploid Transgenic Wheat Lines by Microspore Transformation
Microspores can be induced to develop homozygous doubled haploid plants in a single generation. In the present experiments androgenic microspores of wheat have been genetically transformed and developed into mature homozygous transgenic plants. Two different transformation techniques were investigated, one employing electroporation and the other co-cultivation with Agrobacterium tumefaciens. Di...
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In order to produce doubled haploid lines of bread wheat resistant to strip or yellow rust, three F1 wheat hybrids were crossed using pollens of three maize hybrids. Out of 1071 pollinated florets, success in seed set ranged from 63.1% to 93.3% (mean 78%). Differences in seed set among the crosses were not significant. Embryo formation in the seeds developed on different crosses also varied fro...
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The grass species Brachypodium distachyon has emerged as a model system for the study of gene structure and function in temperate cereals. As a first demonstration of the utility of Brachypodium to study wheat gene promoter function, we transformed it with a T-DNA that included the uidA reporter gene under control of a wheat High-Molecular-Weight Glutenin Subunit (HMW-GS) gene promoter and tran...
متن کاملA wheat HMW glutenin subunit gene reveals a highly repeated structure.
A wheat genomic library was screened with two synthetic oligonucleotides (24 and 25 bases in length) complementary to a partial cDNA clone encoding a glutenin gene [Thompson et al. (1983) Theor. Appl. Genet. 67, 87-96]. Glutenins are large molecular weight aggregated proteins of grain endosperm, and major determinants of bread making quality of wheat. Of the two clones obtained one was fully ch...
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High molecular weight glutenin subunits (HMW-GS) are among the major determinant on wheat bread-making quality. Rapid ultra-performance liquid chromatography (UPLC) methods with high resolution and reproducibility for HMW-GS separation were established in this work. A sample could be completely analyzed in less than 12 min by using the optimized UPLC conditions: gradually increasing elution gra...
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ژورنال
عنوان ژورنال: Czech Journal of Genetics and Plant Breeding
سال: 2012
ISSN: 1212-1975,1805-9325
DOI: 10.17221/6207-cjgpb